2009年4月24日 星期五

抽DNA、plasmid的Kit,內附Buffer配法

試劑配方:capture buffer:4.5M guanidine isothiocyanate
0.5M potassium acetate (pH5.0)
wash buffer:10mM potassium acetate (pH5.0)
16.7uM EDTA (pH8.0)
80% ethanol


Resuspension Solution (or Solution I):50mM Tris-HCl (pH7.5)
10mM EDTA (pH8.0)
100ug/mL RNase A
Lysis Solution (or Solution II):0.2M NaOH
1% SDS
Neutralization Solution (or Solution III):
4.09M guanidine hydrochloride
0.759M potassium acetate
adjust final pH to 4.2 with
glacial acetic acid
Wash Solution:60mM potassium acetate
8.3mM Tris-HCl (pH7.5)
0.04mM EDTA (pH8.0)
60% ethanol